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Nucleic
Acids Research Facilities (NARF)currently include four core laboratories,
the Gene Synthesis Core, the DNA Sequencing Core, the Real Time
PCR Core, and the MicroArraying Core. NARF was completely renovated
during the 2001-02 academic year, and now occupies approximately
2500 square feet including five main laboratories, and several smaller
labs and offices on the fifth floor of Sanger Hall.
The
Gene Synthesis Core maintains a Perceptive Biosystems (now
ABI) DNA synthesizer, HPLC instrumentation for purification, and
a Beckman UV/Vis Spectrophotometer. Standard, fluorescent, biotinylated
or other modified DNA is synthesized for all VCU investigators.
The Gene Synthesis Core is managed by a master's trained molecular
biologist with technical support.
The
DNA Sequencing Core maintains four Automated DNA Sequencers,
including an ABI 3700 Prism 96 Capillary, 3100 Genetic Analyzer
and an Amersham Megabace 96 capillary instrument. Other assets
include 96-well instruments for parallel culture of bacterial
clones and purification of plasmid templates. The DNA Sequencing
Core is managed by a doctoral trained investigator with over 10
years of experience in high throughput DNA sequencing, and is
operated with additional technical support.
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