The
Real Time PCR Core maintains an ABI 7900 TaqManTM Sequence
Detector. The facility performs standard real time PCR for mRNA
quantification and sequence detection PCR for genetic analysis
by SNPs. The RT PCR Core is managed by a master's trained molecular
biologist with technical support.
The
MicroArraying Core maintains an Affymetrix GeneChipTM System
for gene expression analysis using standard (human, mouse, rat,
etc.) arrays. A spotted array system comprised of a BioRobotics
TAS II MicroArraying Spotter and a Packard ScanArray 5000 XL Scanner
is available for generation of custom arrays. The MicroArraying
Core is maintained and operated by a Ph.D.-trained scientist with
technical support.