DNA Sequence Analysis:
DNA Sample
Purity and quality of DNA template play very important roles to obtain good results. The ABI 3100 and 3730 capillary sequencers are very sensitive to template and primer preparation. The templates need to be free of salt and other contaminants which can affect the quality of sequencing data.
- We recommend using QIAGEN plasmid preparation kits or Promega Wizard mini-prep kits. Concentration should be determined by A260/280 OD. Submit 10ul of sample per sequencing reaction. Please see us if you have a big insert >3kb or high GC template.
- We still recommend using QIAGEN or Promega’s PCR Product clean-up kits to clean up your PCR Products. Submit 10ul of sample for sequencing reaction.
- For high throughput sample $5/rxn: The user provides templates in 96 well plates. Core personnel perform the sequencing reaction and analysis data on the ABI 3700 Sequencer and ABI 3100 DNA Analyzer.
- DNA template should be diluted in dH20 not TE buffer. Concentrations as below:
| Plasmid DNA |
100ng/ul |
| PCR Product |
10ng/ul |
| Cosmid, BAC |
200-400ng/ul |
- Please dilute your sample to the concentrations listed. Due to timerestrictions, a fee of $5.00 will be added per dilution.
Fill out a DNA Sequencing Order Form.
If you have any questions, please contact Dr. Yingping Wang.
