The Core Guarantee

Control reactions are run with every sequencing gel. Thus:

1. There is no charge if the control reactions fail.
2. There is no charge if we generated the template (Guaranteed Results).
3. Otherwise, there is a 50% charge for each failed reaction.

Primer

1. We provide a free standard primer with a free charge. Please see the list on the sequencing order form.
2. Standard Primer:
• M13F (-21), M13F (-47), M13R (-22),
• T7P Terminator, T7P Promoter
• SP6, T3
• KS and KS
3. Non-standard primers should be carefully quantified spectrophotometrically (preferably scanned 230-280 nm) and provided with the concentration 20ng/ul. Submit 10ul for each reaction.

Primer Design

A few "rules of thumb" for sequencing primers

1. 18-25 bases in length
2. 40-60% G+C, preferably with 3' G or C
3. No homopolymeric tracks of more than 3 bases
4. Minimize secondary and tertiary structure

Fill out a DNA Sequencing Order Form.
If you have any questions, please contact Dr. Yingping Wang.