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DNA
Microarray Analysis
There
will be a reduced charge of $150.00 per sample for labeling reactions that fail
to give enough labeled RNA for the hybridization procedure. Please check your
RNA with a gel for tight rRNA bands, and with 260/280 readings (≥1.9 in Tris pH8 or ≥1.6 in water).
We have available for use in the NARF a PerkinElmer UV/VIS Spectrophotometer and a BioRad Experion automated
electrophoresis system for RNA analysis. Please contact the NARF for details. If you would like to do your
own labeling reactions we can help you with reagent ordering.
We have computers available in the NARF for microarray analysis. NARF personnel
can introduce you to routine microarray analysis. Analysis software for your
use in the Microarray Core includes Affymetrix GCOS software, Affymetrix Data
Mining Tool and ArrayAssist software from Stratagene. For more detailed help with
statistical setup and analysis of your microarray experiments we can suggest
collaborators from the VCU Department of Biostatistics.
Spotted Arrays
Also available in the Microarray Core is a Biorobotics MicroGrid TAS spotter
for custom spotting of DNA microarrays. We also have a PerkinElmer (Packard)
ScanArray 5000XL scanner. This scanner handles arrays printed on a standard microscope
slide format and has five individual lasers, allowing the scanning of most available
fluorophores.
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