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Real-Time
PCR
Real-Time
PCR analysis detects specific nucleic acid amplification products
as they accumulate in real-time. The ABI Prism® 7900
Sequence Detection System from Applied Biosystems is used to
monitor the increase of the reporter fluorescence during PCR.
The substitution of the old 7700 by the 7900 Sequence Detection
System allowed a decrease in the reaction price since it requires
much lower quantities of reagents. Also, the improved software
gives a much more efficient analysis.
Starting
your RT-PCR experiment
-
Submit the cDNA sequence of the gene of interest
(eukaryotic or prokaryotic)
- Prepare
your samples: ~1ug of DNA or RNA*
*Note: RNA samples must be free of DNA contamination
NARF
will:
- Design
the appropriate primers and probes, including those
for endogenous control
-
Synthesize and purify the selected primers and TaqMan probes
(for lower price and higher quantity of commercially available
reagents)
-
Set up the reactions for RT-PCR in 384-well plates
- Run
the microplates in the ABI PRISM® 7900
- Analyze
and print the data
How
Real-Time PCR Works Sample
Sheet Taqman®
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