diagram taken from TaqMan Gold RT-PCR kit protocol (PE Applied Biosystems)

RT-PCR addresses the problem of end-point analysis, which is commonly seen
in traditional PCR assays. Too much amplification makes it impossible to
quantitate the amount of starting nucleic acid material. RT-PCR monitors the
product accumulation as the PCR amplification proceeds, allowing for higher
sensitivity. Another advantage of real-time PCR is that the PCR tubes don't
need to be opened after the amplification reaction is complete. This prevents
contamination by PCR products and reduces the amount of "false positive"
results. If you need to drop off samples, please fill out a Sample Sheet. Fill out an online Taqman^® Request Form.

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